“肺肠合病”模型大鼠肺与结肠组织CCK-8、CGRP表达的相关性研究中医临床基础专业论文.docx

IV IV “肺肠合病”组肺组织、结肠组织 CGRP 的表达与空白对照组比较有显著差异 （P<0.01）；“肺肠合病”组肺组织、结肠组织 CGRP 的表达与“肺病”对照组 比较有统计学意义（P<0.05）；“肺肠合病”组肺组织 CGRP 的表达与“肠病” 对照组比较有统计学意义（P<0.05）。“肺肠合病”组肺组织 CGRP 的表达与“肠 病”对照组比较有显著差异（P<0.01）。 结论： 1.肺与大肠病理形态学改变的相关性与特异性：实验研究发现，“肺肠合 病”状态下，肺组织和大肠组织的的病理改变较肝、心、脾、肾，以及胃、十 二指肠、空肠、回肠、直肠明显，其提示在“肺肠合病”的情况下，肺与结肠 的相关性较肝、心、脾、肾，以及胃、十二指肠、空肠、回肠、直肠相关高。 2.肺与胃肠组织 CCK-8、CGRP 表达的相关性与特异性：实验发现，“肺肠 合病”状态下，CCK-8、CGRP 表达差异非常显著，由此推测 CCK-8、CGRP 可能为 肺与大肠相联系的共同物质基础，这些物质的相关调控机能，可能是肺与大肠 相互影响的病理学机制之一。 关键词：肺与大肠相表里；肺肠合病；肺病；肠病；CCK-8；CGRP；相关性 PAGE PAGE VI Abstract Purpose: Watch "Fei Chang disease"(asthma together constipation) rat model of liverheart spleen, lung, kidney, and stomach, duodenum, jejunum, ileum, colon, rectum tissue of CCK-8,CGRP expression in Nature, trying to explore the lung and the large intestine provides a reference. Method: This study selected 40 Wistar rats, SPF grade, male. Randomly divided into control group, asthma group (hereinafter referred to as "Fei disease" control group), constipation control group (hereafter referred to as "Chang disease" control group) and asthma co-constipation group (hereinafter referred to as "Fei Chang disease" group), 10 in each group. "Fei disease" of modeling the control group on day 1 and day 8 injection of sensitized asthmatic agent, started 15 days of modeling solution asthmatic OVA inhalation, day 1, continuous spray for 7 days. "Chang disease" control group started from day 1 to give compound diphenoxylate tablets (DC) gavage dose of 10mg/kg, day 1, a total of 11 times. "Fei Chang disease" group, on the modeling day 1 and day 8 injection of sensitized asthmatic agents, modeling 15 day OVA inhalation solution asthmatic, 1 time per day, 7 days a continuous spray ; the same time, starting from day 1 to give, "Fei Chang disease" DC fed rats, the dose 10mg/kg, day 1. Rats were observed during the experiment in general. 21 days of experiment, after atomization, the rats were sacrificed by light microscopy observation of the liver, heart, splee