“肺肠合病”模型大鼠的建立与评价中医临床基础专业论文.docx

大且致密，边缘平直。 7.肺肠菌群检测：与空白对照组比较，“肺肠合病”组大鼠肺部需氧菌和真 菌数升高，厌氧菌变化不明显（P﹤0.01）；肠道需氧菌和大肠杆菌升高，厌氧菌、 真菌、类杆菌和双岐杆菌降低（P﹤0.01）。与“肠病”组比较，“肺肠合病”组 大鼠肺部需氧菌和大肠杆菌数升高（P﹤0.01），厌氧菌变化不明显（P﹤0.05）； 肠道需氧菌和大肠杆菌升高，厌氧菌、真菌、类杆菌和双岐杆菌降低（P﹤0.05）。 与“肺病”组比较，“肺肠合病”组大鼠肺部需氧菌和真菌数升高（P﹤0.01）， 厌氧菌变化不明显（P﹤0.05）；肠道需氧菌和真菌升高（P﹤0.01），厌氧菌、大 肠杆菌类杆菌和双岐杆菌改变不明显（P﹤0.01）。 结论 1.从大鼠一般状态、生活习性的改变，以及相关的生理、病理、生化指标检 测观察，“肺肠合病”模型大鼠的哮喘程度和便秘程度、病理形态学的改变和肺 肠菌群改变，均较单纯“肺病”组和单纯“肠病”组严重，初步证实了“肺肠合 病”复合模型的成功。 2.本研究建立的“肺肠合病”模型大鼠，兼有“肺病”（哮喘）合“肠病” （便秘）的特征，为进一步全面深入研究“肺与大肠相表里”的机理奠定了基础。 3.从宏观表征和微观指标较为客观地评价“肺肠合病”状态和相关病理生理 的变化，部分揭示了“肺”与“肠”可能存在的相互影响，及其影响机制，为“肺 肠合病”模型大鼠的建立与评价提供了依据。 4．“肺肠合病”模型大鼠的建立为研究其他复合动物模型提供了参考和借鉴。 关键词 肺肠合病 模型大鼠 哮喘 便秘 肺与大肠相表里 III ABSTRACT Objective This study attempts to animal experiments, in the "The lung and the 1arge intestine being interior-exteriorly related" under the guidance of TCM(Traditional Chinese Medicine) theory, the establishment of similar rats with clinical prototype, "Fei Chang disease" (asthma together constipation) model, as the "The lung and the 1arge intestine being interior-exteriorly related" Theoretical basis for experimental study, animal model to compare the observed "Fei Chang disease" Characterization and biological indicators of change, and the corresponding changes in the relationship between the evaluation system to establish the model. Methods SPF-class selection of 40 healthy male Wistar rats were randomly divided into 4 groups: control group, "lung disease" (asthma) model group, "bowel disease" (constipation) model group, "Fei Chang disease" (asthma together constipation) model group, 10rats in each group. "lung disease" (asthma) Preparation of model group, the OVA 1mg and aluminum hydroxide 200 mg in normal saline 1ml of fresh gel preparation of sensitization, day 1 and day 8 in rats with bilateral chest, groin, a total of 4 points in the sensitization of the subcutaneous injection of 0.15ml, 0.4ml intraperitoneal injection of a total of 1ml sensitized. The 15th day the rats placed in plexiglass box