富血小板血浆（PRP）在临床中的应用讲义.ppt

Regen PRP 关节炎 延缓进程 难愈创面 甩除顽疾 运动损伤 一针见效 （众多知名运动员的一线之选） Regen PRP 临床应用优势 谢 谢！ * * 09/01/2014 * * Primary fonction of thrombin is to catalyse the conversion of fibrinogen to fibrin; covalent crosslinking The thrombin generation occurs on the surface of activated platelets that form the primary haemostatic plug. A second platelet activation mechanism involves proteolysis of glycoprotein Gp+b-IX-V complex on circulating platelet surface. Thrombin can also activates facto XI (another clotting cascade) requiring anions such as dextran sulphate. But with RegenPRP, we have a scaffold with the same main components, fibrinogen polymerise to form a mesh “the scaffold”, and platelets aggregate to reinforce the clot, delivers growths factors, in addition we have leukocyte which delivers the GF VEGF to enhance the microvascularisation and a multitude of others proteins and vitamin essentials to the new tissue formation; but in this case we have an Autologous scaffold, whish is expended on the patient wound, the patient is its own incubator… Thrombin: two receptors with overlapping functions Is abble to activate plts at concentration as low as 0.1 microM soit 0.01 U/ml, within seconds of the addition of thrombin , the cytosolic Ca2+ concentrate increase 10 fold Plt adhere to fibrinogen & secrete alpha & beta granules Primary haemostasis plts plug clot, secondary fibrin formation Clot haem barrier seal wound tissues scaffold location for cell migration & cell diffrenciation * L’effet cicatrisant du PRP s’explique par la concentration des facteurs cicatrisants qui peuvent manquer dans certaines situations cliniques. Le PRP accélère chaque étape de la cicatrisation représentées ici. Pour l’hemostase: - Le PRP apporte du plasma (fibrinogène & facteurs de coagulation) - Le PRP apporte aussi des plaquettes concentrés 3 à 4 fois qui, en combinaison avec la fibrine, et en l’absence des globules rouges, vont former un caillot particulièrement solide. Les plaquettes relarguent