malditof样品制备方法(含polymer),靶面清洗指导.pdf

MALDI样品制备方法 样品制备流程 The ideal sample preparation in MALDI would be a homogenous layer of small matrix crystals containing a solid solution of the analyte. 待分析物种类/分析目的 选择适当的基质 选择适当的样品靶 选择适当的点靶方法 配置样品/基质溶液 点靶 命名法 Target types: Ground Steel Polished Steel AnchorChip Standard (800µm) AnchorChip Small (400µm) Matrices & HCCA α-Cyano-4-hydroxy cinnamic acid (Bruker Part-No. #201344) additives: 2,5-DHB 2,5-Dihydroxy benzoic acid (Bruker Part no. #201346) sDHB 90:10 mixture of 2,5-DHB and 2-Hydroxy-5-methoxy benzoic acid (Bruker Part no. #209813) 2,5-DHAP 2,5-Dihydroxy actetophenone (Bruker Part No. #231829) SA Trans-3,5-dimethoxy-4-hydroxy cinnamic acid (Bruker Part No. #201345) 1,5-DAN 1,5-Diaminonaphthalene 3-HPA 3-Hydroxy picolinic acid (Bruker Part No. #201224) DAC Diammonium hydrogen citrate NH H PO Ammoniumphosphate, monobasic 4 2 4 TFA Trifluoro acetic acid Solvents: ACN Acetonitrile TA30 30:70 (v/v) Acetonitrile : TFA 0.1% in water TA50 50:50 (v/v) Acetonitrile : TFA 0.1% in water TA85 85:15 (v/v) Acetonitrile : TFA 0.1% in water TA90 90:10 (v/v) Acetonitrile : TFA 0.1% in water TA95 95:5 (v/v) Acetonitrile :