AtPV42a and AtPV42b Redundantly Regulate Reproductive Development in Arabidopsis thaliana 英文参考文献.docVIP
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AtPV42a and AtPV42b Redundantly Regulate Reproductive Development in Arabidopsis thaliana 英文参考文献
AtPV42aandAtPV42bRedundantlyRegulate
ReproductiveDevelopmentinArabidopsisthaliana
LeiFang,XingliangHou,LiYenCandyLee,LuLiu,XiaojingYan,HaoYu*
DepartmentofBiologicalSciencesandTemasekLifeSciencesLaboratory,NationalUniversityofSingapore,Singapore,RepublicofSingapore
Abstract
Background:TheconservedSNF1/AMPK/SnRK1complexesareglobalregulatorsofmetabolicresponsesineukaryotesand
playakeyroleinthecontrolofenergybalance.Althougha-typesubunitsoftheSnRK1complexhavebeencharacterizedin
severalplantspecies,thebiologicalfunctionofb-typeandc-typesubunitsremainslargelyunknown.Here,wecharacterized
AtPV42a and AtPV42b, the two homologous genes in Arabidopsis, which encode cystathionine-b-synthase (CBS) domain-
containingproteinsthatbelongtothePV42classofc-typesubunitsoftheplantSnRK1complexes.
Methodology/Principal Findings: Real-time polymerase chain reaction was performed to examine the expression of
AtPV42aandAtPV42b invarious tissues. Transgenic plantsthatexpressed artificial microRNAstargeting thesetwogenes
were created. Reproductive organ development and fertilization in these plants were examined by various approaches,
includinghistologicalanalysis,scanningelectronmicroscopy,transmissionelectronmicroscopy,andphenotypicanalysesof
reciprocalcrossesbetweenwild-typeandtransgenicplants.WefoundthatAtPV42aandAtPV42bwereexpressedinvarious
tissuesduringdifferentdevelopmentalstages.TransgenicplantswhereAtPV42aandAtPV42bweresimultaneouslysilenced
developedshortersiliquesandreducedseedsets.Suchlowfertilityphenotyperesultedfromderegulationoflatestamen
developmentandimpairmentofpollentubeattractionconferredbythefemalegametophyte.
Conclusions:OurresultsdemonstratethatAtPV42aandAtPV42bplayredundantrolesinregulatingmalegametogenesis
and pollen tube guidance, indicating that the Arabidopsis SnRK1 complexes might be involved in the control of
reproductivedevelopment.
Citation:FangL,HouX,LeeLYC,LiuL,YanX,etal.(2011)AtPV42aandAtPV42bRedundantlyRegulateReproductiveDevelopmentinArabidopsisthaliana .
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