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J. of Supercritical Fluids 55 (2011) 949–954 Contents lists available at ScienceDirect The Journal of Supercritical Fluids journal homepage: cate/supflu Fractionation of thyme (Thymus vulgaris L.) by supercritical fluid extraction and chromatography Mónica R. García-Risco∗, Gonzalo Vicente, Guillermo Reglero, Tiziana Fornari Instituto de Investigación en Ciencias de la Alimentación CIAL (CSIC-UAM) C/Nicolás Cabrera, 9 – Campus de Cantoblanco, 28049 Madrid, Spain a r t i c l e i n f o Article history: Supercritical fluid chromatography (SFC) was employed to fractionate thyme (Thymus vulgaris L.) extracts, Received 28 July 2010 which were obtained by supercritical carbon dioxide extraction of thyme leaves. First, different supercriti- Received in revised form 6 October 2010 cal extracts were produced at 313 K and at different pressures (15, 30 and 40 MPa). Thymol, a monocyclic Accepted 6 October 2010 terpenoid with recognized eptic, gesic and anti-infl tory properties, was identified and fied in the different samples by GC–MS. Then, the supercritical extracts were fractionated by semi- : preparative SFC, and different conditions such as pressure, temperature and amount of cosolvent Supercritical fluid chromatography