分子生物学资料：Chapter 10_Questions.ppt

? 2014 Pearson Education, Inc. MOLECULAR BIOLOGY OF THE GENE ? 2014 Pearson Education, Inc. Seventh Edition Watson ? Baker ? Bell ? Gann ? Levine ? Losick Questions by Bryan Jennings and James Lodolce Chapter 10 Site-Specific Recombination and Transposition of DNA 课后作业 2014-12-18 1. “Conservative site-specific recombination (CSSR) can involve the insertion, deletion, or inversion of DNA segments depending on the orientation of the recombination sites.How would you classify the type of CSSR observed in ? bacteriophage? How would you classify Hin recombinationin Salmonella? insertion and deletion for ? phage; inversion for Hin insertion for ? phage; deletion for Hin inversion for ? phage; insertion for Hin inversion for ? phage; insertion and deletion for Hin 2. Which of the following is a characteristic of the recombination mechanism employed by tyrosine recombinases? A branched DNA intermediate is formedafter first strand exchange. All four strands of two DNA molecules are cleaved before strand exchange. Recombination occurs using a single recombinase subunit. Recombinase dimers in the covalent protein–DNA intermediate rotate to complete strand exchange. 3. Which of the following components are required for integration of bacteriophage ? genome into the host bacterial genome? attB, attP, ? Int, IHF attB, attP, ? Int, Xis attL, attR, ? Int, IHF attB, attP, ? Int 4. Which of the following statements about transposon target-site duplications is NOT true? They are the recognition sequences for transposases. They result from the transposase-mediated strand transfer reaction. They are organized as direct repeats in all classes of transposable elements. The length of the duplicated sequence reveals the distance between the sites attacked and cut on each strand in the target DNA. 5. Which of the following is a key characteristic of the active sites in certain transposases and integrases? conserved aspartate, aspartate, andglutamate residues conserved arginine