分子生物学资料：Chapter 9_Questions.ppt

? 2014 Pearson Education, Inc. MOLECULAR BIOLOGY OF THE GENE ? 2014 Pearson Education, Inc. Seventh Edition Watson ? Baker ? Bell ? Gann ? Levine ? Losick Questions by Bryan Jennings and James Lodolce Chapter 9 Homologous Recombination at the Molecular Level 课后作业 2014-12-11 1. “Splice” or crossover products can form after the resolution of Holliday junctions during homologous recombination between two nearly identical parental duplexes. Which of the following answers best describes the characteristics of these recombination products? Each product contains heteroduplex DNA in the region of recombination, while the genes in the flanking regionsbecome reassorted. Each product contains homoduplex DNA in the region of recombination, while the genes in the flanking regions become reassorted. Each product contains heteroduplex DNA in the region of recombination, while the genes in the flanking regions do not become reassorted. One product contains heteroduplex DNA in the region of recombination, while the other contains homoduplex DNA in this same region. The genes in the flanking regions of both products become reassorted. 2. Which of the following answers best describes all of the agents that can introduce double-stranded breaks (DSBs) in most bacteria and/or eukaryotes? DSBs in bacteria can be introduced by ionizing radiation orby failed replication forks; the same is true in eukaryotes.DSBs in eukaryotes are also introduced by the protein Spo11. DSBs in bacteria and eukaryotes can be introduced by ionizing radiation or by failed replication forks. DSBs in both bacteria and eukaryotes can be introduced by ionizing radiation, failed replication forks, and by the protein Spo11. DSBs in bacteria and eukaryotes can be introduced by ionizing radiation or by failed replication forks. DSBs in bacteria are also introduced by the protein Spo11. 3. What are the functions of RecBCD in E. coli ? Facilitate homologous recombination by generating3? single-stranded tail