Differential Effects of Nitrogenous Fertilizers on Methane-Consuming英文清晰.pdf

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Feb. 2006, p. 1346–1354 Vol. 72, No. 2 0099-2240/06/$08.00 0 doi:10.1128/AEM.72.2.1346–1354.2006 Copyright © 2006, American Society for Microbiology. All Rights Reserved. Differential Effects of Nitrogenous Fertilizers on Methane-Consuming Microbes in Rice Field and Forest Soils†‡ Santosh R. Mohanty,1 2 2 1 § Paul L. E. Bodelier, * Virgilio Floris, and Ralf Conrad Max Planck Institute for Terrestrial Microbiology, Department of Biogeochemistry, Karl-von-Frisch-Str., 35043 Marburg, Germany, 1 and Netherlands Institute of Ecology (NIOO-KNAW), Centre for Limnology, Department of Microbial Wetland Ecology, Rijksstraatweg 6, 3631 AC Nieuwersluis, The Netherlands2 Received 13 September 2005/Accepted 18 November 2005 The impact of environmental perturbation (e.g., nitrogenous fertilizers) on the dynamics of methane fluxes from soils and wetland systems is poorly understood. Results of fertilizer studies are often contradictory, even within similar ecosystems. In the present study the hypothesis of whether these contradictory results may be explained by the composition of the methane-consuming microbial community and hence whether methanotro- phic diversity affects methane fluxes was investigated. To this end, rice field and forest soils were incubated in microcosms and supplemented with different nitrogenous fertilizers and methane concentrations. By labeling the methane with 13C, diversity and function could be coupled by analyses of phospholipid-derived fatty acids (PLFA)