PAAS37℃热稳定性与储存缓冲液的关系-SpringerStaticContentServer.docVIP

Supplementary Materials for Striking effects of storage buffers on apparent half-lives of the activity of Pseudomonas aeruginosa arylsulfatase Yuwei Li 1,a, Xiaolan Yang1,a, Deqiang Wang1, Xiaolei Hu1, Mei Yuan1, Jun Pu1, Chang-Guo Zhan2, Fei Liao1,* 1Key Laboratory of Medical Laboratory Diagnostics of the Education Ministry, College of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China; 2Molecular Modeling and Biopharmaceutical Center and Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, 789 South Limestone Street, Lexington, KY 40536, U.S.A. ___________________________________________________________________________ a These authors contributed equally to this work. *Corresponding author, Tel: +86-23 E-mail: Liao F, liaofeish@, liaofeish@ Note S1, DNA and the amino acid sequence of PAAS with 6His at the N-terminus Translation of PAAS DNA.txt(1-1647) Total amino acid number: 547, MW=61179 Max ORF starts at AA pos 1(may be DNA pos 1) for 546 AA(1638 bases), MW=61050 1 CATATGCACCACCACCACCACCACAGCAGCGGCAGCAAACGCCCCAACTTCCTGGTGATC 1 (RE)M H H H H H H S S G S K R P N F L V I 61 GTCGCCGACGACCTGGGCTTCTCCGATATCGGCGCCTTCGGCGGCGAGATCGCCACGCCG 21 V A D D L G F S D I G A F G G E I A T P 121 AACCTCGACGCCCTGGCCATCGCCGGCCTGCGCCTGACCGACTTCCACACCGCCTCGACC 41 N L D A L A I A G L R L T D F H T A S T 181 TGCTCGCCGACCCGCTCGATGCTGCTCACCGGCACCGACCACCACATCGCCGGGATCGGC 61 C S P T R S M L L T G T D H H I A G I G 241 ACCATGGCCGAGGCGCTGACCCCGGAACTGGAAGGCAAGCCGGGTTACGAAGGGCATCTC 81 T M A E A L T P E L E G K P G Y E G H L M72 301 AACGAGCGCGTGGTGGCGCTGCCGGAGCTGCTCCGCGAGGCCGGCTACCAGACCCTCATG 101 N E R V V A L P E L L R E A G Y Q T L M 361 GCCGGCAAGTGGCACCTCGGTCTGAAGCCGGAACAGACGCCCCATGCACGCGGTTTCGAG 121