Pulse Oximeter Laboratory - The University of （脉搏血氧计实验室,大学的）.pdf

Pulse Oximeter Laboratory Urs Utzinger, Assistant Professor U of A, modified 2002-6. Table of Contents Pulse Oximeter Laboratory 1 Table of Contents 1 With material from 1 Introduction 2 Oxygen saturation 2 Optical absorption measurement 2 Optical properties of hemoglobin 4 [RHb] and [HbO2] in mixture 6 Arterial versus venous blood 6 Measurement wavelengths 9 Pulse oximeter math 10 Hardware 13 Photo detectors principle of operation 14 Other Components 20 Laboratory instructions 20 Week 1: 20 Week 2: 21 Optional: 21 If it does not work: 21 With material from • John G. Webster, /bme/courses/bme310.html (Bioinstrumentation) • Dissertation Swiss Federal Institute of Technology, Jürg Wyser, Measurement of the arterial oxygen saturation in the airplane • Hamamatsu technotes: • Optical properties of Hemoglobin and blood: /spectra/hemoglobin/index.html Introduction Oxygen saturation Hemoglobin is a protein and the main component of red blood cells. Hemoglobin transports oxygen from the lungs, where oxygen tension (partial pressure of oxygen) PO2 is high, to the tissues, where oxygen tension is low. Oxygen saturation, SO , is defined as 2 the ratio of the amount of bound oxygen to the total oxygen capacity. [HbO2 ] HbO2 SAT [RHb] +[HbO2 ] where [HbO2] is the concentration of oxyhemoglobin, and [RHb] is the concentration of deoxyhemoglobin. If the hemoglobin molecule is bound to oxygen then one has oxy- hemoglobin or [HbO ]. If the hemoglobin molecule is bound to carbon mono