大肠杆菌杀菌曲线.pdf

2013 3rd Teaching Seminars on Higher Education Science and Engineering Courses The exploration and practice on the comprehensive experiment in advanced experiment course of microbiology Effect of the physiological states of bacterial population on the result of antibiotic susceptibility test Na Liu, Nana Zhou, Wei Qian, Huaiqiang Zhang* (College of Life Science, Shandong University, Jinan, China, 250100) * Corresponding author: Tel:+86-0531 E-mail:zhq@ Abstract: In this study, the physiological states of Escherichia coli CVCC249 under batch culture are determined by the numerical differential method and nonlinear fitting method. The antibiotic susceptibility of E.coli CVCC249 at different physiological states is accurately characterized with different concentrations of antibiotics based on the concentration-killing curve method. The test studied the effect of physiological states of the bacterial population on the antibacterial agents’s killing power. It will help to provide the basis for the clinical use of drugs and the improvement of the method of antibiotic susceptibility test. Keywords: Escherichia coli; numerical differential method; physiological state; CKC method; antibiotic susceptibility 微生物大实验课程综合性实验项目的探索与实践 —细菌菌群生理状态对抗菌药物敏感性检测实验结果的影响 刘娜, 周娜娜, 钱卫，张怀强* （山东大学 生命科学学院 山东 济南 250100 ） * 通讯作者：Tel:+86-0531 E-mail:zhq@ 摘要：首先基于数值微分和非线性方程拟合方法，准确确定了大肠杆菌 E.coli CVCC249 在限制性分批培 养下的菌群生理状态。进一步通过浓度杀菌曲线（CKC）法，定量表征了处于不同生理状态下的菌群对抗 菌药物恩诺沙星敏感性的差异，进而阐明菌群生理状态对抗菌药物杀菌力的影响。研究结果可望为药敏性 实验方法的改进和临床抗菌药物的合理使用，提供理论依据。 关键词：大肠杆菌；数值微分；生理状态；浓度杀菌曲线（CKC）法；抗生素敏感性 1 前言 当前，由于抗生素的大量使用，导致细菌耐药性/抗药性问题日趋严重，从而造成抗生素的治疗效果 大为降低[1-4] 。因此，在临床医学上，合理科学地使用抗生素，对避免因抗生素滥用导致细菌抗药性的产 生，起着至关重要性的作用。获得稳定而又准确的药敏检测结果，是合理科学地使用抗生素的重要依据[5]。