PTEN能负调控前列腺癌PC-3细胞Raf-1磷酸化.pdf

21 PTEN能负调控前列腺癌PC-3细胞Raf-1磷酸化 1 1* 2 1 1 3 孙健玮 王剑松 刘子超 杨峻峰 马 辉 郑立民 1.昆明医科大学第二附属医院泌尿外科 （昆明 650101 ）; 2.昆明学院生命科学与技术系; 3.昆明医科大学第五附属医院泌尿外科 摘要 目的 探讨 PTEN基因表达变化对Raf-1磷酸化的影响。方法 用Western blot方法分别检测PTEN基因 过表达和干扰后前列腺癌PC-3细胞Raf-1 的磷酸化水平。结果 PTEN基因过表达后，PC-3细胞的磷酸化Raf-1 减少 61.1%；PTEN基因干扰后，磷酸化Raf-1增加75.1%。结论 PTEN可负调控前列腺癌PC-3细胞Raf-1 的磷酸化。 关键词 PTEN磷酸水解酶; Raf激酶类; 前列腺肿瘤 doi: 10.3969/j.issn.1008-0848.2014.01.005 中图分类号 R 737.25 PTEN downregulates in the level of Raf-1 phosphorylation in prostate cancer cell PC-3 1 1* 2 1 1 3 Sun Jianwei , Wang Jiansong , Liu zichao , Yang Junfeng , Ma Hui , Zheng Limin 1.Department of Urology, the Second Affiliated Hospital of Kunming Medical University, Kunming 650101, China; 2. Department of Biologican Science and Technology, Kunming University; 3. Department of Urology, the Fifth Affiliated Hospital of Kunming Medical University Corresponding author: Wang Jiansong, E-mail: Jiansongwang@ Abstract Objective To investigate the effects of PTEN expression on level of Raf-1 phosphorylation in prostate cancer cells. Methods The level of Raf-1 phosphorylation was detected by western blot in prostate cancer cells with PTEN gene overexpression or PTEN gene knockdown. Results The level of Raf-1 phosphorylation decreased by 61.1% in PC-3 cells with PTEN gene overexpressio