Amino Acid Analyser.PPT

Amino Acid Analyser Amino Acid Analyser Products Ion exchange chromatography Application An example of application Amino Acid Analyser AMINO ACID ANALYSER (AAA) Amino Acid Analyser is a specifically configured system optimised for the analysis of free amino acids. PRINCIPLE The system utilises ion-exchange chromatography incorporating post column reaction with ninhydrin and subsequent detection in the visible region spectrum.  Amino Acid Analyser Biochrom AAA: AccQ Tag The AccQTag Method is based on a derivatizing reagent developed specifically for amino acid analysis. Waters AccQFluor Reagent (6-aminoquinolyl-N-hydrozysuccinimidyl carbamate, or ACQ) is an N-hydroxysuccinimide-activated hetrocyclic carbamate, a new class of amine-derivatizing compounds. Chromatography Open Column Chromatography Thin Layer Chromatography Liquid Chromatography Ion exchange chromatography Amino acid analyer High Performance Liquid Chromatography Protein analysis Determination of Amino Acid Composition of Proteins 1) break down the polypeptide chain into its constituent amino acids , hydrolysis (boiling in 6 N HCl) 2) separate the resulting free amino acids according to type, 3) measure the quantities of each amino acid. chromatography Protein analysis Methods of protein purification Differential centrifugation Diferential precipitaion by soulbility difference Column procedures Preparative gel-exclusion chromatography Column chromatography with protein binding Ion-exchange chromatography Affinity chromatography High-performance Liquid Chromatography Electrophoretic methods Protein analysis Determination of Amino Acid Sequence of Proteins Sequenators (Edman degradation) phenylisothiocyanate + tetrapeptide - phenylthiocarbamoyltetrapeptide mild hydrolysis phenylthiohydantion derivative of NH2-terminal amino acid + original peptide minus NH2-terminal residue Ion exchange chromatography - ????? ????? ????(p